The Red Devils are not familiar to feature in this competition in the early stages. However, their seventh place finish has forced them to feature in the second round this season.
Louis van Gaal will be searching for his first competitive win as United manager after the first two matches ended in a defeat and a draw against Swansea City and Sunderland respectively.
Confirmed lineup for the match will be out at 7pm BST:
Manchester United: De Gea, M Keane, Evans, Vermijl, James, Powell, Janko, Anderson, Hernandez, Kagawa, Welbeck
MK Dons: Martin; Baldock, Lewington (c), Kay, McFadzean; Alli, Potter; Bowditch, Reeves, Carruthers; Grigg.