During the Sevilla-Getafe game
Puerta collapsed after jogging back towards his own goal 35 minutes into the opening league game of the season at the Sanchez Pizjuan on Saturday.
Team mates and medical staff rushed to his side and were seen preventing him from swallowing his tongue as he lost consciousness.
He was able to walk from the pitch but doctors said that he collapsed again in the changing rooms and had to be given cardiac resuscitation before being taken to the Virgen del Rocio hospital.
He was placed in intensive care and doctors said on Tuesday his condition had deteriorated before his death.
Sevilla’s fixtures postponed
Sevilla were due to play AEK Athens in the second leg of a third round Champions League qualifier on Tuesday but the match has been postponed until Monday 3 September.
As a consequence, the league match against Osasuna that was scheduled for next Monday has been postponed.